Journal: Cells

Article Title: IFN-λ Modulates the Migratory Capacity of Canine Mammary Tumor Cells via Regulation of the Expression of Matrix Metalloproteinases and Their Inhibitors

doi: 10.3390/cells10050999

Figure Lengend Snippet: Primary antibodies used for Western blot analysis.

Article Snippet: phospho-STAT1 , rabbit , 1:1000 , Biorbyt orb214616.

Techniques: Western Blot

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Overexpression of NLRP12 enhances macrophage immune response and alleviates herpes simplex keratitis

doi: 10.3389/fcimb.2024.1416105

Figure Lengend Snippet: NLRP12 enhances antiviral functions through IL-18 downstream JAK-STAT signaling. (A) Protein levels of STAT1, p-STAT1, STAT4, pSTAT4 were assessed at 8h, 12h and 24h post-infection in both groups. Western blot bands were normalized to β-actin and compared against controls (n=3 per group). (B) Immunofluorescence images demonstrating MHC II + cell staining. All of the data are representative of at least three independent experiments. Data are presented as the mean ± SEM. Statistical differences were determined using two-way ANOVA (A) . ns, not significnat, *P <0.05, ***P <0.001, ****P <0.0001.

Article Snippet: The membranes were then blocked with Y-Tec 5min Rapid Blocking Buffer (YWB0501, Yoche, China) and incubated overnight with primary antibodies against NLRP12 (1:1000, YT7568, Immunoway, USA), HSV-gB (1:500, sc-56987, Santa Cruz, USA), HSV-gD (1:500, sc-21719, Santa Cruz, USA), CASP1 (1:2000, 81482–1-RR, Proteintech, China), N-GSDMD (1:2000, ab215203, Abcam, UK), clv-CASP1 (1:500, AY0406, Abways, China), IL-18 (1:500, A1115, ABclonal, China), IL-1β (1:1000, ab254360, Abcam, UK), STAT1 (1:1000, CY5227, Abways, China), STAT1 (Phospho-S727) (1:1000, BM4541, Boster, USA), STAT4 (1:1000, BA0622–2, Boster, USA), Phospho-STAT4 (Tyr693) (1:1000, CY6503, Abways, China), and β-actin (1:20000, 81115–1-RR, Proteintech, China).

Techniques: Infection, Western Blot, Immunofluorescence, Staining

Journal: International Journal of Molecular Sciences

Article Title: Human Interleukin 23 Receptor Induces Cell Apoptosis in Mammalian Cells by Intrinsic Mitochondrial Pathway Associated with the Down-Regulation of RAS/Mitogen-Activated Protein Kinase and Signal Transducers and Activators of Transcription factor 3 Signaling Pathways

doi: 10.3390/ijms141224656

Figure Lengend Snippet: The mitogenic signaling pathways are repressed by IL-23R in 293ET cells. ( a ) Western blot analysis was performed using anti ERK, anti-phosphorylated ERK, anti STAT1, anti-phosphorylated STAT1, anti Akt, and anti-phosphoryled Akt antibodies. The tranfected Myc-IL-23R after 48 h transfection was visualized by anti Myc antibody. Beta-actin gene expression was served as internal control; and ( b ) Western blot analysis was performed using anti STAT3 and anti-phosphorylated STAT3 antibodies. β-actin gene expression was served as internal control. In both ( a ) and ( b ), the relative intensity of individual band was calculated based on the expression of β-actin by using the Quantity One program.

Article Snippet: Rabbit anti-STAT1 and Rabbit anti-p-STAT1 were purchased from Bioworld Technology Inc. (Bioworld, CA, USA).

Techniques: Western Blot, Transfection, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Human Interleukin 23 Receptor Induces Cell Apoptosis in Mammalian Cells by Intrinsic Mitochondrial Pathway Associated with the Down-Regulation of RAS/Mitogen-Activated Protein Kinase and Signal Transducers and Activators of Transcription factor 3 Signaling Pathways

doi: 10.3390/ijms141224656

Figure Lengend Snippet: The intracellular signaling pathways are altered upon the overexpression of IL-23R in HeLa cells. ( a ) Western blot analysis was performed anti-phosphorylated extracellular regulated protein kinases (p-ERK), anti ERK, anti STAT3 and anti-phosphorylated STAT3 antibodies. β-actin gene expression was served as internal control; and ( b ) Western blot analysis was performed using using anti-phosphoryled Akt, anti Akt, anti-phosphorylated STAT1 and anti STAT1 antibodies. β-actin gene expression was served as internal control. In both ( a ) and ( b ), the relative intensity of the individual bands were calculated based on the expression of β-actin by using the Quantity One program.

Article Snippet: Rabbit anti-STAT1 and Rabbit anti-p-STAT1 were purchased from Bioworld Technology Inc. (Bioworld, CA, USA).

Techniques: Over Expression, Western Blot, Expressing